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fgf7 (fibroblast growth factor 7)  (PeproTech)


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    PeproTech fgf7 (fibroblast growth factor 7)
    Fgf7 (Fibroblast Growth Factor 7), supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fgf7+(fibroblast+growth+factor+7)/basic+fibroblast+growth+factor/pmc11961725-205-18-28
    Average 90 stars, based on 1 article reviews
    fgf7 (fibroblast growth factor 7) - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Breast cancer-on-chip for patient-specific efficacy and safety testing of CAR-T cells.
    Article Snippet: Afterwards, 280 mL of culture medium (AdvDMEM/F12+++ supplemented with 10% (v/v) conditioned medium from L-WRN cells (ATCC-CRL-3276),57 5 nM Heregulinß-1 (100-03; PeproTech, NJ, USA), 5 ng/mL fibroblast growth factor 7 (FGF7) (100-19; PeproTech), 20 ng/mL fibroblast growth factor 10 (FGF10) (100-26; PeproTech), 5 ng/mL epidermal growth factor (EGF) (AF-100-15; PeproTech), 500 nM A83-01 (2939; Tocris, Wiesbaden, Germany), 5 mM Y27632 (72034; STEMCELL Technologies), 500 nM SB-202190 (S7067; Sigma-Aldrich), 2% B27 Supplement (17504-044; Thermo Fisher Scientific), 1.25 mM N-acetyl-cysteine (A9165; Sigma-Aldrich), 5 mM nicotinamide (N0636; Sigma-Aldrich)) was added to each well.

    Article Title: Glucose Transporters Are Key Components of the Human Glucostat
    Article Snippet: On day 4, DE cells were rinsed with PBS and exposed to S1/2 medium supplemented with 50 ng/mL keratinocyte growth factor (fibroblast growth factor 7 [FGF7]; PeproTech, 100-19) and 0.25 mmol/L vitamin C (Sigma-Aldrich, A4544) for 2 days.

    Article Title: Single cell transcriptomic comparison between mouse embryonic pancreas and pancreatic organoids generated from mouse embryonic stem cell-derived mesoderm and pancreatic progenitors
    Article Snippet: On day 4, medium was changed to BE1 with 50ng/mL keratinocyte growth factor (KGF also known as FGF7, Peprotech) for 2 days.

    Article Title: Zebrafish Avatar testing preclinical study predicts chemotherapy response in breast cancer
    Article Snippet: Cancer cells were resuspended in Mix1 with human EGF (Epithelial Growth Factor) (5 ng mL −1 , Peprotech), FGF7 (Fibroblast Growth Factor 7) (5 ng mL −1 , Peprotech), FGF10 (Fibroblast Growth Factor 10) (20 ng mL −1 , Peprotech), A83-01 (Selective inhibitor of TGF-βRI, ALK4 and ALK7) (500 nM, Peprotech) and Neuroregulin-1 (5 nM, Peprotech).

    Article Title: cccDNA Surrogate MC-HBV–Based Screen Identifies Cohesin Complex as a Novel HBV Restriction Factor
    Article Snippet: PHH cells were cultured on rat tail collagen-coated plates with Advanced DMEM/F12 (Thermo Scientific, Waltham, MA) plus 500 ng/mL R-spondin1 (Peprotech, Rocky Hill, NJ), B27 (minus vitamin A), 1.25 mmol/L N-acetylcysteine (Sigma, St. Louis, MO, USA), 10 mmol/L nicotinamide (Sigma), 50 ng/mL epidermal growth factor (Peprotech), 10 nmol/L gastrin (Sigma), 3 μmol/L CHIR99021 (Sigma), 50 ng/mL hepatocyte growth factor (HGF) (Peprotech), 100 ng/mL fibroblast growth factor-7 (FGF7) (Peprotech), 100 ng/mL FGF10 (Peprotech), 20 ng/mL transforming growth factor α (Peprotech), and 2 μmol/L A83-01 (Tocris Bioscience, Bristol, UK).

    Cell Culture:

    Article Title: Anti-frizzled antibodies and methods of use
    Article Snippet: .. Growth factors commonly used in cell culture medium include epidermal growth factor (EGF, (Peprotech), Transforming Growth Factor-alpha (TGF-alpha, Peprotech), basic Fibroblast Growth Factor (bFGF, Peprotech), brain-derived neurotrophic factor (BDNF, R&D Systems), Hepatocyte Growth Factor (HGF) and Keratinocyte Growth Factor (KGF, Peprotech, also known as FGF7). ..

    Article Title: Compositions comprising R-Spondin (RSPO) surrogate molecules
    Article Snippet: .. Growth factors commonly used in cell culture medium include epidermal growth factor (EGF, (Peprotech), Transforming Growth Factor-alpha (TGF-alpha, Peprotech), basic Fibroblast Growth Factor (bFGF, Peprotech), brain-derived neurotrophic factor (BDNF, R&D Systems), Human Growth Factor (HGF) and Keratinocyte Growth Factor (KGF, Peprotech, also known as FGF7). ..

    Concentration Assay:

    Article Title: Generation of human pluripotent stem cell derived functional beta cells showing a glucose-dependent mitochondrial respiration and two-phase insulin secretion response
    Article Snippet: .. Stage 2 (3 Days): Aggregates were treated for two days with MCDB-131 medium containing 2.7 g/1000 ml sodium bicarbonate, and supplemented with 0.5% FAF-BSA, 1× GlutaMAXTM, 4.5 mM D-glucose to obtain a concentration of 10 mM D-glucose, 0.25 mM ascorbic acid, and 50 ng/ml fibroblast growth factor 7 (“FGF7”; PeproTech, Catalog No. AF-100-19). ..



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    The high level of E 2 hyperactivates FGF-FGFR-ERK signaling cascade. A Relative mRNA expression levels of FGFs and their receptors in endometrial explants treated with or without the high level of E 2 ( n = 5). B Immunohistochemical analysis of <t>FGF7</t> in endometrial explants treated with or without the high level of E 2 . Scale bar: 20 μm. C Western blotting analysis of FGF7 level in endometrial explants treated with or without the high level of E 2 ( n = 7). The upper panel shows the quantification of FGF7 protein level. D and E Western blotting analysis of phosphorylation levels of FGFR ( D ) and ERK ( E ) in endometrial explants treated with or without the high level of E 2 ( n = 7). The upper panel shows the quantification of pFGFR and pERK levels. Data are presented as the mean ± SEM. * P < 0.05, ** P < 0.01. E 2 , estradiol; FGF , fibroblast growth factor; FGF7, fibroblast growth factor 7; FGFR, fibroblast growth factor receptor; ERK, extracellular signal-regulated kinase; pFGFR, phosphorylated FGFR; pERK, phosphorylated ERK
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    The high level of E 2 hyperactivates FGF-FGFR-ERK signaling cascade. A Relative mRNA expression levels of FGFs and their receptors in endometrial explants treated with or without the high level of E 2 ( n = 5). B Immunohistochemical analysis of <t>FGF7</t> in endometrial explants treated with or without the high level of E 2 . Scale bar: 20 μm. C Western blotting analysis of FGF7 level in endometrial explants treated with or without the high level of E 2 ( n = 7). The upper panel shows the quantification of FGF7 protein level. D and E Western blotting analysis of phosphorylation levels of FGFR ( D ) and ERK ( E ) in endometrial explants treated with or without the high level of E 2 ( n = 7). The upper panel shows the quantification of pFGFR and pERK levels. Data are presented as the mean ± SEM. * P < 0.05, ** P < 0.01. E 2 , estradiol; FGF , fibroblast growth factor; FGF7, fibroblast growth factor 7; FGFR, fibroblast growth factor receptor; ERK, extracellular signal-regulated kinase; pFGFR, phosphorylated FGFR; pERK, phosphorylated ERK
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    The high level of E 2 hyperactivates FGF-FGFR-ERK signaling cascade. A Relative mRNA expression levels of FGFs and their receptors in endometrial explants treated with or without the high level of E 2 ( n = 5). B Immunohistochemical analysis of FGF7 in endometrial explants treated with or without the high level of E 2 . Scale bar: 20 μm. C Western blotting analysis of FGF7 level in endometrial explants treated with or without the high level of E 2 ( n = 7). The upper panel shows the quantification of FGF7 protein level. D and E Western blotting analysis of phosphorylation levels of FGFR ( D ) and ERK ( E ) in endometrial explants treated with or without the high level of E 2 ( n = 7). The upper panel shows the quantification of pFGFR and pERK levels. Data are presented as the mean ± SEM. * P < 0.05, ** P < 0.01. E 2 , estradiol; FGF , fibroblast growth factor; FGF7, fibroblast growth factor 7; FGFR, fibroblast growth factor receptor; ERK, extracellular signal-regulated kinase; pFGFR, phosphorylated FGFR; pERK, phosphorylated ERK

    Journal: Journal of Animal Science and Biotechnology

    Article Title: Dietary supplementation with N -acetyl-L-cysteine ameliorates hyperactivated ERK signaling in the endometrium that is linked to poor pregnancy outcomes following ovarian stimulation in pigs

    doi: 10.1186/s40104-024-01109-1

    Figure Lengend Snippet: The high level of E 2 hyperactivates FGF-FGFR-ERK signaling cascade. A Relative mRNA expression levels of FGFs and their receptors in endometrial explants treated with or without the high level of E 2 ( n = 5). B Immunohistochemical analysis of FGF7 in endometrial explants treated with or without the high level of E 2 . Scale bar: 20 μm. C Western blotting analysis of FGF7 level in endometrial explants treated with or without the high level of E 2 ( n = 7). The upper panel shows the quantification of FGF7 protein level. D and E Western blotting analysis of phosphorylation levels of FGFR ( D ) and ERK ( E ) in endometrial explants treated with or without the high level of E 2 ( n = 7). The upper panel shows the quantification of pFGFR and pERK levels. Data are presented as the mean ± SEM. * P < 0.05, ** P < 0.01. E 2 , estradiol; FGF , fibroblast growth factor; FGF7, fibroblast growth factor 7; FGFR, fibroblast growth factor receptor; ERK, extracellular signal-regulated kinase; pFGFR, phosphorylated FGFR; pERK, phosphorylated ERK

    Article Snippet: To further investigate the downstream pathways, explants were cultured in the presence of 200 ng/mL of recombinant fibroblast growth factor 7 (FGF7; Invitrogen), 10 μmol/L FGFR inhibitor BGJ398 (Selleck, Houston, USA), or 100 nmol/L ERK inhibitor PD0325901 (Selleck).

    Techniques: Expressing, Immunohistochemical staining, Western Blot

    The high level of E 2 induces overproliferation of endometrium via FGF-FGFR-ERK signaling cascade. A Immunohistochemical analysis of PCNA in endometrial explants treated with or without recombinant FGF7. Scale bar: 20 μm. B Western blotting analysis of PCNA in endometrial explants treated with or without recombinant FGF7 ( n = 7). The upper panel shows the quantification of PCNA level. C and D Western blotting analysis of phosphorylation levels of FGFR ( C ) and ERK ( D ) in endometrial explants treated with or without recombinant FGF7 ( n = 7). The upper panels show the quantification of pFGFR and pERK levels. E and F Western blotting analysis of phosphorylation levels of FGFR ( E ) and ERK ( F ) in endometrial explants treated with the high level of E 2 alone or in combination with FGFR inhibitor ( n = 5). The upper panels show the quantification of pFGFR and pERK levels. G Western blotting analysis of phosphorylation levels of ERK in endometrial explants treated with the high level of E 2 alone or in combination with ERK inhibitor. The upper panel shows the quantification of the pERK level ( n = 5). H Immunohistochemical analysis of PCNA in endometrial explants treated with or without FGFR inhibitor or ERK inhibitor. Scale bar: 20 μm. I Western blotting analysis of PCNA in endometrial explants treated with or without FGFR inhibitor or ERK inhibitor ( n = 5). The upper panel shows the quantification of PCNA level. Data are presented as the mean ± SEM. * P < 0.05, ** P < 0.01. PCNA, proliferating cell nuclear antigen; FGF7, fibroblast growth factor 7; FGFR, fibroblast growth factor receptor; ERK, extracellular signal-regulated kinase; pFGFR, phosphorylated FGFR; pERK, phosphorylated ERK; E 2 , estradiol

    Journal: Journal of Animal Science and Biotechnology

    Article Title: Dietary supplementation with N -acetyl-L-cysteine ameliorates hyperactivated ERK signaling in the endometrium that is linked to poor pregnancy outcomes following ovarian stimulation in pigs

    doi: 10.1186/s40104-024-01109-1

    Figure Lengend Snippet: The high level of E 2 induces overproliferation of endometrium via FGF-FGFR-ERK signaling cascade. A Immunohistochemical analysis of PCNA in endometrial explants treated with or without recombinant FGF7. Scale bar: 20 μm. B Western blotting analysis of PCNA in endometrial explants treated with or without recombinant FGF7 ( n = 7). The upper panel shows the quantification of PCNA level. C and D Western blotting analysis of phosphorylation levels of FGFR ( C ) and ERK ( D ) in endometrial explants treated with or without recombinant FGF7 ( n = 7). The upper panels show the quantification of pFGFR and pERK levels. E and F Western blotting analysis of phosphorylation levels of FGFR ( E ) and ERK ( F ) in endometrial explants treated with the high level of E 2 alone or in combination with FGFR inhibitor ( n = 5). The upper panels show the quantification of pFGFR and pERK levels. G Western blotting analysis of phosphorylation levels of ERK in endometrial explants treated with the high level of E 2 alone or in combination with ERK inhibitor. The upper panel shows the quantification of the pERK level ( n = 5). H Immunohistochemical analysis of PCNA in endometrial explants treated with or without FGFR inhibitor or ERK inhibitor. Scale bar: 20 μm. I Western blotting analysis of PCNA in endometrial explants treated with or without FGFR inhibitor or ERK inhibitor ( n = 5). The upper panel shows the quantification of PCNA level. Data are presented as the mean ± SEM. * P < 0.05, ** P < 0.01. PCNA, proliferating cell nuclear antigen; FGF7, fibroblast growth factor 7; FGFR, fibroblast growth factor receptor; ERK, extracellular signal-regulated kinase; pFGFR, phosphorylated FGFR; pERK, phosphorylated ERK; E 2 , estradiol

    Article Snippet: To further investigate the downstream pathways, explants were cultured in the presence of 200 ng/mL of recombinant fibroblast growth factor 7 (FGF7; Invitrogen), 10 μmol/L FGFR inhibitor BGJ398 (Selleck, Houston, USA), or 100 nmol/L ERK inhibitor PD0325901 (Selleck).

    Techniques: Immunohistochemical staining, Recombinant, Western Blot

    Effect of various treatments on hWJMSCs-Sec toward growth factor proteins level. A) KGF, B) HGF, C) HB-EGF, D) PDGF, E) EGF. The data were presented as mean ± standard deviation. Different superscript letters on Figure A (a, b, c, cd, d, e, f), and B, C, D, E (a, b, c, d, e, f, g) showed significant differences among treatments at p<0.05 (Data was analyzed using ANOVA followed by Tukey HSD post hoc test). Samples included hWJMSCs-Sec (S), Freeze-dried hWJMSCs-Sec (FDS), Freeze-dried Medium Basal (FDMB), Freeze-Dried hWJMSCs-Sec Gel (FDSG), and Freeze-dried Gel Based (FDGB). Number on samples indicate the ratio of gel to hWJMSCs-Sec, 1) 6 g : 3 ml , 2) 6 g : 4.5 ml , and 3) 6 g : 6 ml .

    Journal: Avicenna Journal of Medical Biotechnology

    Article Title: The Potential of Human Wharton’s Jelly Mesenchymal Stem Cells Secretome Based Topical Gel for Therapeutic Application

    doi: 10.18502/ajmb.v16i4.16739

    Figure Lengend Snippet: Effect of various treatments on hWJMSCs-Sec toward growth factor proteins level. A) KGF, B) HGF, C) HB-EGF, D) PDGF, E) EGF. The data were presented as mean ± standard deviation. Different superscript letters on Figure A (a, b, c, cd, d, e, f), and B, C, D, E (a, b, c, d, e, f, g) showed significant differences among treatments at p<0.05 (Data was analyzed using ANOVA followed by Tukey HSD post hoc test). Samples included hWJMSCs-Sec (S), Freeze-dried hWJMSCs-Sec (FDS), Freeze-dried Medium Basal (FDMB), Freeze-Dried hWJMSCs-Sec Gel (FDSG), and Freeze-dried Gel Based (FDGB). Number on samples indicate the ratio of gel to hWJMSCs-Sec, 1) 6 g : 3 ml , 2) 6 g : 4.5 ml , and 3) 6 g : 6 ml .

    Article Snippet: The Human KGF (E-EL-H0092), HGF (E-EL-H0084), PDGF (E-EL-H2211), EGF (E-EL-H0059), and HB-EGF (E-EL-H2667) level were assayed using the Elabscience kit, based on manufacturing protocols.

    Techniques: Standard Deviation

    Proposed mechanism of hWJMSCs-Sec gel as a wound healing agent. * The treatment of hWJMSCs-Sec gel into the wounded skin can regulate some growth factors such as KGF, HGF, HB-EGF, PDGF, and EGF. The upregulation of these growth factors can induce the production of collagen, which plays a crucial role to regenerate tissue formation. The high antioxidant hWJMSCs-Sec gel also has the ability to scavenge free radicals, and inhibit the excessive inflammation that can cause irritation, redness, and pain in the wound.

    Journal: Avicenna Journal of Medical Biotechnology

    Article Title: The Potential of Human Wharton’s Jelly Mesenchymal Stem Cells Secretome Based Topical Gel for Therapeutic Application

    doi: 10.18502/ajmb.v16i4.16739

    Figure Lengend Snippet: Proposed mechanism of hWJMSCs-Sec gel as a wound healing agent. * The treatment of hWJMSCs-Sec gel into the wounded skin can regulate some growth factors such as KGF, HGF, HB-EGF, PDGF, and EGF. The upregulation of these growth factors can induce the production of collagen, which plays a crucial role to regenerate tissue formation. The high antioxidant hWJMSCs-Sec gel also has the ability to scavenge free radicals, and inhibit the excessive inflammation that can cause irritation, redness, and pain in the wound.

    Article Snippet: The Human KGF (E-EL-H0092), HGF (E-EL-H0084), PDGF (E-EL-H2211), EGF (E-EL-H0059), and HB-EGF (E-EL-H2667) level were assayed using the Elabscience kit, based on manufacturing protocols.

    Techniques: